WOUND HEALING ACTIVITY OF POLY HERBAL FORMULATION IN ALBINO RATS USING EXCISION WOUND MODEL, INCISION WOUND MODEL, DEAD SPACE WOUND MODEL AND BURN WOUND MODEL

Authors

  • M A Muqeem Nasir Department of Pharmacology, AZAD college of pharmacy, Moinabad, R. R. District, Telangana-501504
  • N.Lal Mahammed Department of Pharmacology, AZAD college of pharmacy, Moinabad, R. R. District, Telangana-501504
  • S. Roshan, Mohd Wasif Ahmed Department of Pharmacology, AZAD college of pharmacy, Moinabad, R. R. District, Telangana-501504

Keywords:

poly herbal formulation, wound healing, Excision wound, Hydroxyproline, Incision wound

Abstract

The present study was conducted to investigate the wound healing activity of the selected Indian medicinal plants. The present study was designed to evaluate wound healing activity of poly herbal formulation (Curcuma longa, Eclipta alba, and Tridax procumbens) in Wistar albino rats using excision wound model, incision wound model, dead space wound model and burn wound model at two different dose levels of 250 and 500 mg/kg. The suitable gel formulation has been selected by cellophane membrane study to apply topically. The following parameters were checked in the present study to assess the effects of poly herbal formulation extract on wound healing. Collagenation phase of healing (for this incision and dead space wound model)Wound contraction (excision wound model and burn wound model) Epithelization phases of healing (excision wound model and burn wound model). Wound healing was monitored on Days 3, 6, 9 and 14 and histological evaluation was carried out on the samples. In the excision wound and burn wound model, rat treated with poly herbal formulation leaf extract showed significant difference in period of epithelisation,wound contraction 50%. In incision wound model, poly herbal formulation extract treated rat showed significant increase in breaking strength. In dead space wound model, poly herbal formulation shows significant increase in breaking strength, dry weight and hydroxyproline content of the granulation tissue. It can be concluded that poly herbal formulation topically (5% and 10% gel formulation) and orally (250 mg and 500 mg/Kg body weight) have wound healing activity, which may be due to its epithelization, thereby justifying the traditional claim.

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Published

2016-03-15

How to Cite

Nasir, M. A. M., Mahammed, N. ., & Ahmed, S. R. M. W. . (2016). WOUND HEALING ACTIVITY OF POLY HERBAL FORMULATION IN ALBINO RATS USING EXCISION WOUND MODEL, INCISION WOUND MODEL, DEAD SPACE WOUND MODEL AND BURN WOUND MODEL. International Journal of Research and Development in Pharmacy & Life Sciences, 5(2), 2080-2087. Retrieved from https://www.ijrdpl.com/index.php/ijrdpl/article/view/222