A comparative evaluation of total antioxidant capacity of saliva in children with and without Gingivitis

Authors

  • Bharat Kumar Department of Pediatric and Preventive Dentistry, King George’s Medical University, Lucknow, India
  • Afroz Alam Ansari Department of Pediatric and Preventive Dentistry, King George’s Medical University, Lucknow, India
  • Sanchit Tiwari Department of Biochemistry, King George’s Medical University, Lucknow, India
  • Mohd Waseem Department of Biochemistry , King George’s Medical University, Lucknow, India
  • Seema Singh Department of Biochemistry, King George’s Medical University, Lucknow, India
  • A.A. Mahdi Department of Biochemistry, King George’s Medical University, Lucknow, India
  • Rakesh Kumar Chak Department of Pediatric and Preventive Dentistry, King George’s Medical University, Lucknow, India

DOI:

https://doi.org/10.21276/IJRDPL.2278-0238.2019.8(3).7-11

Keywords:

Diabetic nephropathy, Gene polymorphism, Glucose transporter gene, Restriction fragment length poly-morphism, Type 2 Diabetes melitus

Abstract

Gingivitis is a reversible and non-destructive form of periodontal disease. Oxidative stress contributes in the pathogenesis of periodontal diseases5. The oxidative stress has been implicated as one of the important contributory etiologic factors in many of the oral inflammatory pathologies including gingivitis. This research analyzed the "Total antioxidant capacity" (TAC) of biological fluids including saliva. The present cross-sectional study was conducted to evaluate the total antioxidant capacity (TAC) of saliva in children with/ without gingivitis and its relation with Age and Gender. For measuring the TAC of saliva: Cayman's Antioxidant Assay Kit was used and Gingival Index Measured through The Gingival Index (Löe and Silness, 1963). The results were analyzed using descriptive statistics and making comparisons between cases and control by using SPSS software version 20. In this result, mean TAC of saliva in case children group was found lower 0.203 ± 0.053 compared to control children group was higher 0.236 ± 0.048. While, in male and female children of aged 3-5 years were found antioxidant activity (TAC) lower in compared to control groups. But among males aged 6-13 years it was found that the mean antioxidant capacity of saliva in case group was 0.259 ± 0.040 while in control group it was 0.295 ± 0.026. The TAC of saliva in males was found high compared to female. A weak negative correlation was found between the TAC and gingival index. In conclusion TAC decreases in children with gingivitis compared to healthy children. The gingivitis was more observed in female leading to lower TAC value

Downloads

Download data is not yet available.

References

Albandar, Jasim M., and Thomas E. Rams. Global epidemiology of periodontal diseases: an overview. Periodontology 2000. 2002; 29(1): 7-10.

Mariotti A. Dental Plaque-Induced Gingival Diseases. Annals of Periodontology. 1999;4(1):7-17.

The American Academy of Periodontology, "Periodontal diseases of children and adolescences," Journal of Periodontology, 1996; 67: 57-62.

Clerehugh V and Tugnait A. Diagnosis and management of periodontal diseases in children and adolescents. Periodontology 2000. 2001; 26(1):146-168.

Chapple I and Matthews J. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontology 2000. 2007; 43(1):160-232.

Kiy iyoshima, Tamotsu, Norio Enoki, Ieyoshi Kobayashi, Takako Sakai, Kengo Nagata, Hiroko Wada, Hiroaki Fujiwara, Yukiko Ookuma, and Hidetaka Sakai. Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts. International Journal of Molecular Medicine. 2012; 30(5):1007-1012.

Chapple I. Reactive oxygen species and antioxidants in inflammatory diseases. Journal of Clinical Periodontology. 1997; 24(5):287-296.

Halliwell B and Whiteman M. Measuring reactive species and oxidative damagein vivoand in cell culture: how should you do it and what do the results mean. British Journal of Pharmacology. 2004; 142(2):231-255.

Bartold P, Wiebkin and O, Thonard J. The effect of oxygen-derived free radicals on gingival proteoglycans and hyaluronic acid. Journal of Periodontal Research. 1984; 19(4):390-400.

Battino M, Ferreiro M, Gallardo I, Newman H and Bullon P. The antioxidant capacity of saliva. Journal of Clinical Periodontology. 2002; 29(3):189-194.

Chapple, I.L.C., G.I. Mason, I. Garner, J. B. Matthews, G. H. Thorpe, S. R. J. Maxwell, and T. P. Whitehead. Enhanced Chemiluminescent Assay for Measuring the Total Antioxidant Capacity of Serum, Saliva and Crevicular Fluid. Annals of Clinical Biochemistry. 1997; 34(4):412-421.

Löe H and Silness J. Periodontal disease in pregnancy I. Prevalence and severity. Acta odontologica scandinavica. 1963; 21(6):533-51.

Battino M, Ferreiro MS, Gallardo I, Newman HN and Bullon P. The antioxidant capacity of saliva. J Clin Periodontol 2002; 29(3):189-194.

Zhang, Taowen, Oleh Andrukhov, Hady Haririan, Michael Müller-Kern, Shutai Liu, Zhonghao Liu, and Xiaohui Rausch-Fan. Total Antioxidant Capacity and Total Oxidant Status in Saliva of Periodontitis Patients in Relation to Bacterial Load. Frontiers in cellular and infection microbiology. 2015; 5:97.

Ahmadi-Motamayel F, Goodarzi MT, Jamshidi Z and Kebriaei R. Evaluation of Salivary and Serum Antioxidant and Oxidative Stress Statuses in Patients with Chronic Periodontitis: A Case-Control Study. Frontiers in physiology. 2017; 8:189.

Baser U, Gamsiz-Isik H, Cifcibasi E, Ademoglu E and Yalcin F. Plasma and salivary total antioxidant capacity in healthy controls compared with aggressive and chronic periodontitis patients. Saudi medical journal. 2015; 36(7):856.

Tamaki N, Yoshino F, Fukui M, Hayashida H, Yoshida A, Kitamura M, Iwasaki T, Furugen R, Kawasaki K, Nakazato M and Maeda T. Relationship among salivary antioxidant activity, cytokines, and periodontitis: the Nagasaki Island study. Journal of clinical periodontology. 2015; 42(8):711-8.

Furuta M, Ekuni D, Irie K, Azuma T, Tomofuji T, Ogura T and Morita M. Sex differences in gingivitis relate to interaction of oral health behaviors in young people. Journal of periodontology. 2011; 82(4):558-65.

Pepe H, Balci ?S, Revan S, Akalin PP and Kurto?lu F. Comparison of oxidative stress and antioxidant capacity before and after running exercises in both sexes. Gender medicine. 2009; 6(4):587-95.

Matsson L and Goldberg P. Gingival inflammation at deciduous and permanent teeth an intra?individual comparison. Journal of clinical periodontology. 1986; 13(8):740-2.

How to cite this article:

Kumar B, Tiwari S, Ansari AA, Waseem M, Singh S, Mahdi AA, Chak RK. A comparative evaluation of total antioxidant capacity of saliva in children with and without Gingivitis. Int. J. Res. Dev. Pharm. L. Sci. 2019; 8(3): 7-11. doi: 10.13040/IJRDPL.2278-0238.8(3).7-11

This Journal is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

Published

2019-06-15

How to Cite

Kumar, B. ., Ansari, A. A. ., Tiwari, . S. ., Waseem, M. ., Singh, S., Mahdi, A. ., & Chak, R. K. (2019). A comparative evaluation of total antioxidant capacity of saliva in children with and without Gingivitis. International Journal of Research and Development in Pharmacy & Life Sciences, 8(3), 07-11. https://doi.org/10.21276/IJRDPL.2278-0238.2019.8(3).7-11

Issue

Vol. 8 No. 3 (2019): Volume 8 Issue 3 , May - June (2019)

Section

Original Article
Creative Commons License

This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

Most read articles by the same author(s)

1 2 > >>