ISOLATION AND CHARACTERIZATION OF CHEMICAL CONSTITUENTS OF ASPARAGUS RACEMOSUS AS MARKERS
Keywords:
Asparagus racemosus, Marker Compounds, Column Chromatography, TLC, Shatavarins, MPLC, HPLC, 1H NMR, 13C NMR.Abstract
In the present study an attempt was made to isolate and characterize the phytoconstituents from Asparagus racemosus and utilize them as marker compounds. Powdered root of the drug was extracted and the butanolic extract was used for the isolation process. Isolation of the phytoconstituents was done by Column chromatography using gradient elution with different stationary phases and mobile phases of varying polarity. Two compounds were isolated and characterized by NMR spectroscopy. A HPLC method was developed to estimate the isolated two compounds. The simplicity of isolation and HPLC analysis of the compounds may be utilized as markers in standardization of preparations containing Asparagus racemosus.
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