REGULATION OF INFLAMMATION VIA ASA LOADED PROTEIN NANOPARTICLES
Keywords:
Gelatin, aspirin, anti-inflammatory activity, paw edema, corneal retention.Abstract
The axiom of the current research was to formulate a protein based nanoparticles loaded with a NSAID to regulate chronic deformity which occurs due to inflammation as in case of rheumatoid arthritis, diabetic retinopathy etc. Two step desolvation method was espousedto prepare the formulation with gelatin; being biodegradable and non-toxic material as drug delivery vehicle and the drug of choice; aspirin which shows efficient results in the suppression of rheumatoid arthritis and diabetes induced eye inflammation. Effect of various experimental variables like temperature, stirring speed and concentration of cross-linking agent (glutaraldehyde) were observed. The entrapment efficiency of nanoparticles containing varied concentration of cross linker (glutaraldehyde) shows entrapment of drug in range of 51.74 -56.53% and the particle size was found in the range of 192 nm to 402 nm.In vitro release profile was applied on various kinetic models in order of zero order, first order, Higuchi equation and Korsmeyer-Peppas equation. Formulation-II treated reduced carrageenan induced paw edema by 35.29% and Formulation-I treated reduced the inflammation by 31.37% as compared to control in non-diabetic rats. Ex vivo study on goat eyes exposed satisfactory result of drug penetration and retention at corneal tissues. The Formulation was stable for all parameters after 60 days.
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