IN VITRO CORNEAL PERMEATION OF CELECOXIB FROM OIL DROPS

Authors

  • Ajit Kumar Acharya Royal College of Pharmacy and Health Sciences, Berhampur-76002 (Ganjam), Odisha, India
  • Gitanjali Mishra P.G. Department of Zoology, Berhampur University, Berhampur- 760007 (Ganjam), Odisha, India
  • Dipak Kanti Majumdar Delhi Institute of Pharmaceutical Sciences and Research, Formerly College of Pharmacy, (University of Delhi), Pushp Vihar, Sector-III, New Delhi-10017, India

Keywords:

Celecoxib, solubility, partition coefficient, corneal permeation, corneal hydration

Abstract

The objective of present investigation was to study the in vitro permeation characteristics of celecoxib from oil drops through freshly excised goat corneas. Celecoxib ophthalmic solutions (0.3 to 1.0% w/v in arachis, castor and mustard oil or 0.3 to 0.5% w/v in olive, sesame and 0.3% w/v in sunflower oil) were prepared with or without benzyl alcohol (0.5% v/v). Permeation studies were conducted by placing 1 ml oil formulation on cornea (0.64 cm2) fixed between donor and receptor compartment of an all-glass modified Franz diffusion cell and the drug permeation in receptor (containing 10 ml bicarbonate ringer, pH 7.4 at 370C) was measured by spectrophotometer at 248 nm, after 120 minutes. The study was designed with paired corneas i.e. one cornea of an animal received formulation without benzyl alcohol while the contralateral cornea received formulation with benzyl alcohol. The maximum corneal permeation (0.099 mg) was obtained from 0.5% (w/v) celecoxib drops in sesame oil with benzyl alcohol, while minimum (0.016 mg) from 0.3% (w/v) formulation in castor oil without benzyl alcohol. Addition of benzyl alcohol, a preservative, to oil drops increased permeation of celecoxib from each oil drops. This could be due to increased partitioning of celecoxib in the aqueous phase in the presence of benzyl alcohol. Corneal hydration obtained with all the formulations was between 75 to 80% indicating no corneal damage. In conclusion, increasing celecoxib concentration increased the corneal permeation from oils but among all, celecoxib (0.5% w/v) in sesame oil containing benzyl alcohol showed the maximum permeation.

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References

Polansky J, Weinreb R. Pharmacology of the Eye. New York: Springer. 460–583: 1984.

Hersh PS, Rice BA, Baer JC, Wells PA, Lynch SE, Mcguigan LJB, Foster S. (1990) Arch. Ophthalmol.108:577-583.

Searle AE, Pearce JL, Shaw DE. (1990) Br. J. Ophthalmol. 74:19-21.

Copper LA, Bergamini MVW, Leopold IH. (1980) Arch. Ophthalmol. 98:1102-1105.

Solomen KD, Cheetham JK, Degryse R., Brint SF, Rosenthal A. (2001) Ophthalmology. 108:331-337.

Kraff MC, Saunders DR, Mcguigan L, Rannan MG. (1990) Arch. Ophthalmol. 108:380-383.

Kim SJ, Flach AJ, Jampol LM. (2010) Surv Ophthalmol. 55:108–133.

Reddy R, Kim SJ. (2011) Clin Ophthalmol. 5:751–758.

Kim SJ, Hubbard GB. (2008) Arch Ophthalmol. 126:1203–1208.

Paulson S, Vaughn M, Jessen S, Lawal Y, Gresk C, Yan B, Maziasz T, Cook C, Karim A. (2001) J. Pharmacol. Exp. Ther. 297:638–645.

Ozsoy Y, Gungor S, Cevher E. (2004) Farmaco. 59:563-566.

Wiederholt M, Kossendrup D, Schulz W, Hoffmann F. (1986) Invest Ophthalmol. Vis Sci. 27:519-524.

Banker GS, Rhodes CS: “Modern Pharmaceutics”, Marcel Dekker, New York, Ed. 4th, 2007.

Tilmouth T, Briscoe J. (1984) Med. J. Australia. 140 (2):119.

Malhotra M, Majumdar DK. (1997) Indian J. Exp. Biol. 35: 1324-1330

Ahuja M, Dhake AS, Majumdar DK. (2006) Yakugaku Zasshi. 126(12):1369-1375.

Maurice DM, Riley MV. (1970) Biochemistry of the Eye, Academic Press, London, 6-16.

Longer MA, Robinson JR: “Remington's Pharmaceutical Sciences,” Mack Publishing Company, Easton, PA, Ed. 19th, 1990.

Published

2015-01-15

How to Cite

Acharya, A. K. ., Mishra, . G. ., & Majumdar, D. K. . (2015). IN VITRO CORNEAL PERMEATION OF CELECOXIB FROM OIL DROPS. International Journal of Research and Development in Pharmacy & Life Sciences, 5(1), 1955-1962. Retrieved from https://www.ijrdpl.com/index.php/ijrdpl/article/view/207