EVALUATION OF ANTINOCICEPTIVE ACTIVITY OF ETHANOLIC EXTRACT OF AZADIRACHTA INDICA LEAVES
Keywords:
Azadirachta indica leaves, formalin test, acetic acid induced writhing, tail immersionAbstract
Objective: The present study was carried out to investigate the antinociceptive activities of the ethanol extract of Azadirachta indica leaves (Neem) on experimental animal models at two different dose levels of 200mg/kg and 400mg/kg.
Materials and methods: Swiss albino mice (25-30g) of either sex were selected for this study. Each group consisted of six animals. Antinociceptive activity of ethanol extract was evaluated by well-established models like formalin test; acetic acid induced writhing and tail immersion method in mice.
Results: Oral administration of the ethanol extract of Azadirachta indica leaves (200,400mg/kg) significantly (P<0.05) attenuated the early and late phase of formalin induced paw licking response in mice. Further it exhibited significant inhibition of (P<0.05) acetic acid induced writhing test for both the dosage and increased the withdrawal latency time in tail immersion test at the dose of (200, 400mg/kg).
Conclusion: These observations suggest that, the ethanol extract of Azadirachta indica leaves has potent antinociceptive activities, which explains the basis of its use in traditional medicine to manage acute and chronic pain. Further investigations are required to identify the active principle responsible for this beneficial effect.
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