EVALUATION OF ANTI-INFLAMMATORY POTENTIAL OF ETHANOLIC EXTRACT OF THE LEAVES OF RHIZOPHORA MUCRONATA, A SUNDERBAN MANGROVE
Keywords:
Inflammation, antioxidant, mangrove, RhizophoraAbstract
Objective: Biochemically unique mangrove plants are recognized worldwide for valuable medicinal actions. Experimental investigations of these pharmacological activities are needed for elucidation of cost effective new therapeutic compound with better efficacy and less toxicity. Present study evaluated the in-vitro and invivo anti-inflammatory activity of the ethanolic extract of the leaves of Sunderban mangrove, Rhizophora mucronata Lam. Method: The leaves were collected from Sunderban, identified and extracted with ethanol. The phytochemical analysis of the extract, preliminary antioxidant studies were done and impact of the extract (RME) on nitric oxide production was assessed in lipopolysaccharide (LPS) stimulated RAW264.7 macrophage cell line. The anti-inflammatory activity was studied in Carrageenan induced acute and Freund's adjuvant induced chronic inflammatory experimental animal model. Result: Present study showed that RME significantly scavenged free radicals and significantly inhibited nitrite accumulation in cell line. Quercetin is an important flavonoid present in the extract. The extract reduced the rodent paw edema in acute and chronic inflammation models. In the chronic study, the extract in 100mg/kg dosage orally markedly reduced (44.44%) paw edema than control, compared to the standard Diclofenac sodium (33.33%). Conclusion: Study revealed the significant free radical scavenging and anti-inflammatory activity of the ethanolic extract of Rhizophora mucronata Lam. leaves. Further studies including isolation of active principle will assess the mechanisms involved in the observed activities.
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