Optimization of Diesel oil degrading Bacterial strains at various culture parameters
DOI:
https://doi.org/10.21276/IJRDPL.2278-0238.2017.6(6).2840-2844Keywords:
Hydrocarbon, Optimization, Bioremediation, parametersAbstract
Environmental pollution with petroleum has been recognized as one of the most serious current problems. Petroleum hydrocarbons are a combination of hydrocarbons obtained from reservoirs of crude petroleum. The petroleum hydrocarbons contain aliphatic hydrocarbons and aromatic hydrocarbons. The most common petroleum hydrocarbons polluting environment are the gasoline, diesel and fuel oils. Numerous microorganisms could use petroleum as only source of carbon as energy for metabolic activities, and these microorganisms are broadly present and distributed in nature. Bioremediation is the use of microorganism metabolism to remove pollutants. Bioremediation is the biological process involving living microorganisms to remove contaminants or pollutants from soil or water. It involves the use of microbes to detoxify and humiliate environmental contaminants. The present study is carried out by optimization of diesel oil degrading microbes from diesel oil polluted sites. Bacillus sp and Enterobacter hormaechei were isolated according to Bergey’s manual and by 16s rRNA gene sequence. Further the optimization was carried out for various culture parameters such as various hydrocarbon concentration, pH, temperature and for best carbon and nitrogen sources. The present study suggests that Bacillus sp and Enterobacter hormaechei were able to degrade diesel oil at pH 7, 300 C and preliminary hydrocarbon concentration of 1%.
Downloads
References
Bossert I & Bartha R, 1984, The fate of petroleum in soil ecosystems in Petroleum Microbiology, edited by R M Atlas. Macmillan, New York. Pp. 435 – 473.
Baraniecki C A et al, 2002, Characterization of Sphingomonas sp. Ant17, an aromatic hydrocarbon-degrading bacterium isolated from Antarctic soil. Microb Ecol. 42, 958 – 963.
Delille D & Delille B, 2000, field observations on the variability of crude oil impact in indigenous hydrocarbon degrading bacteria from sub-Antarctic intertidal sediments. Mar Environ Res, 49, 403 – 417.
Leahy J G & Colwell R R, 1990. Microbial degradation of hydrocarbons in the environment. Microbiol Rev, 54, 305 – 315.
Margestin R & Schinner F, 1998. Low temperature bioremediation of a wastewater contaminated with anionic surfactants and fuel oil. Appl Microbiol Biotechnol, 49, 482 486.
Haritash AK, Kaushik CP. Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs): a review. J Hazard Mater. 2009; 169:1–15. doi: 10.1016/j.jhazmat.2009.03.137.
Whang L-M, Liu P-WG, Ma C-C, Cheng S-S. Application of rhamnolipid and surfactin for enhanced diesel biodegradation-Effects of pH and ammonium addition. J Hazard Mater. 2009; 164:1045–1050.
Luo Q, Zhang J-G, Shen X-R, Sui X, Fan Z-Q. Characterization of a novel diesel oil-degrading pseudomonas sp. strain F4. Fresenius Environ Bullet. 2013; 22:689–697.
Sathishkumar M, Binupriya AR, Baik S-H, Yun S-E. Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas. Clean-Soil Air Water. 2008; 36:92–96.
Mnif I, Sahnoun R, Ellouze-Chaabouni S, Ghribi D. Evaluation of B. subtilis SPB1 biosurfactants’ potency for diesel-contaminated soil washing: optimization of oil desorption using Taguchi design. Environ Sci Pollut Res. 2014; 21:851–861.
Luo Q, Xian-Rong S, Jian-Guo Z, Zheng-Qiu F, Ying H. Isolation, identification and biodegradation ability of diesel oil degrading Pseudomonas sp. strain C7 from bilge water. Afr. J Microbiol. 2012; 6:1033–1040.
Prathima Devi M, Venkateswar Reddy M, Juwarkar A, Nageswara Sarma P, Venkata Mohan SR. Effect of co-culture and nutrients supplementation on bioremediation of crude petroleum sludge. Clean-Soil Air Water. 2011; 39:900–907.
Vasudevan, N. and Rajaram, P. (2001) Bioremediation of Oil Sludge-Contaminated Soil. Environment International, 26, 409-411.
Whyte, L. G., L. Bourbonnie`re, and C. W. Greer. 1997. Biodegradation of petroleum hydrocarbons by psychotropic Pseudomonas strains possessing both alkane (alk) and naphthalene (nah) catabolic pathways. Appl. Environ. Microbiol. 63:3719–3723.
Alexander M (1994), Biodegradation and Bioremediation, San diego: Academic press, pp. 43 – 48.
Published
How to Cite
Issue
Section
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
.